SeaDataCloud Flow Cytometry Standardised Cluster Names
The microphytoplankton group is defined by cells with a size range between 20 and 200 microns. No staining is required to distinguish microphytoplankton by flow cytometry. This group is discriminated thanks to its forward scatter and red fluorescence signals larger than those of the other groups. Due to the low volumes analyzed by flow cytometry, this group is not always accurately quantified when cells are not abundant. It is possible to distinguish this group from nanophytoplankton (2-20 microns in diameter) when the forward scatter signal is calibrated to detect the forward scatter channel corresponding to the 20 micron limit in size. On cytometers equipped with an image in flow device, pictures are also used to measure cell size. Chains or colonies may outpass flow cytometry analysis depending on instrument performance (tubing size, pulse shape analysis or not). If large cryptophytes or coccolithophores are observed, they will be considered in a separate group thanks to their distinguishable optical properties.
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